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menA [2020-06-18 10:04:44]
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menA [2020-06-18 10:04:44]

probable 1,4-dihydroxy-2-naphthoate octaprenyltransferase
locus
BSU_38490
pI
9.32
mw
33.68 kDa
protein length
311 aa Sequence Blast
gene length
936 bp Sequence Blast
function
biosynthesis of menaquinone
product
probable 1,4-dihydroxy-2-naphthoate octaprenyltransferase
essential
yes
ec
2.5.1.74
synonyms
ywaB,ipa-6d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.8|Biosynthesis of menaquinone]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,950,726 3,951,661

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • 1,4-dihydroxy-2-naphthoate + all-trans-polyprenyl diphosphate + H+ --> 2-demethylmenaquinol + CO2 + diphosphate (according to UniProt)

    • Protein family

  • MenA family (single member, according to UniProt)

    • Structure

  • [PDB|3BYV] (from ''Toxoplasma gondii'', 30% identity in a 25% fragment) [Pubmed|19197235]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|34DBBDD76A0B36867E5116FF17391DF70062F745|menA]
    description
    [Pubmed|9353933]

    regulation

  • the leader mRNA is processed by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-B217 (ywaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1216 NBRP B. subtilis, Japan]

    • References

  • 9353933,19197235,29794222